| R64 | Sylwia Mikos (CHI) def. Kylie Wilson (WIT) 6-3, 6-0 |
| R64 | Eleni Dakos (KENW) def. Sarah Sgambati (JCU) 6-3, 6-3 |
| R64 | Sofia Strupovets (LFC) def. Kimbeley Peterson (PRIN) 6-1, 6-0 |
| R64 | Katherine Johnston (WASHUW) def. Taylor Goldman (RHIT) 6-1, 6-1 |
| R64 | Lily McCloskey (CWR-W) def. Amber Edmonds (WASHUW) 6-2, 6-4 |
| R64 | Caitlyn Hanulikova (AUGIE) def. Borunda, Cristina (MILLIKIN) 6-3, 6-1 |
| R64 | Bassett, Natalie (NCCW) def. Addison Brannon (GRU) 6-0, 6-0 |
| R64 | Journee White (WEB) def. Freya Rahm (OBEW) 6-2, 6-2 |
| R64 | Shianna Guo (CHI) def. Lillia Walter (OTT) 6-0, 6-1 |
| R64 | Annika Weeber (HOPE) def. Caroline Lopez (DENW) 2-6, 6-2, 6-1 |
| R64 | Allaire Berl (KENW) def. Katherine Taylor (TRANSY) 6-2, 6-0 |
| R64 | Jenna Bell (ONU) def. Katie King (DPUW) 6-3, 6-0 |
| R64 | Karen Gao (WASHUW) def. Maddie Hurley (KAL) 6-1, 6-3 |
| R64 | Anna-Maria Huebel (AUGIE) def. Madeline Kavenagh (JCU) 6-3, 6-0 |
| R64 | Radha Patel (CWR-W) def. Olivia Colby (FRAN) 6-0, 6-1 |
| R64 | Janaki Jagannathan (WOOW) def. Emilee Bassett (TRINE) 6-2, 6-2 |
| R64 | Lydia Zafeiri (OBEW) def. Melaina Tippery (HAN) 6-2, 6-0 |
| R64 | Eleanor Archer (WASHUW) def. Ella Frantz (AU-W) 6-0, 6-0 |
| R64 | Amanda Lopykinski (HOPE) def. Valerie Doherty (DPUW) 6-4, 6-2 |
| R64 | Oliwia Mikos (CHI) def. Margaret Bugnacki (LFC) 6-0, 6-0 |
| R64 | Erin Storrer (OTT) def. Shadia Amado Aguad (KENW) 6-1, 6-2 |
| R64 | Dasha Chistyakova (DENW) def. Wynne Aldrich (RHIT) 6-1, 6-0 |
| R64 | Sarena Biria (CHI) def. Jamie Hurst (ONU) 6-1, 6-1 |
| R64 | Hannah Kassaie (CWR-W) def. Juliana Pullen (KAL) 6-0, 6-1 |
| R64 | Otilia Oita (WOOW) def. Grace Gardner (TRANSY) 7-5, 6-1 |
| R64 | Nicole Geller (CHI) def. Maria Herrera (FRAN) 6-3, 6-3 |
| R64 | Peyton Oda (DENW) def. Caroline Sproule (WIT) 6-4, 6-3 |
| R64 | Penados, Abigail (NCCW) def. Markus, Emily (MILLIKIN) 6-0, 6-2 |
| R64 | Lanie Carder (GRU) def. Rachel Pinnick (HAN) 1-6, 6-2, 6-0 |
| R64 | Nina Moravek (WASHUW) def. S. Hernandez Cisnero (PRIN) 6-0, 6-0 |
| R64 | Katalina Wang (CWR-W) def. Elina Locane (TRINE) 6-1, 6-0 |
| R64 | Eleni Lazaridou (KENW) def. Megan Long (AU-W) 6-0, 6-0 |
| R32 | Sylwia Mikos (CHI) def. Eleni Dakos (KENW) 6-2, 6-0 |
| R32 | Katherine Johnston (WASHUW) def. Sofia Strupovets (LFC) 7-6 (7-4), 6-0 |
| R32 | Lily McCloskey (CWR-W) def. Caitlyn Hanulikova (AUGIE) 6-2, 6-0 |
| R32 | Bassett, Natalie (NCCW) def. Journee White (WEB) 6-1, 6-0 |
| R32 | Shianna Guo (CHI) def. Annika Weeber (HOPE) 6-1, 6-1 |
| R32 | Allaire Berl (KENW) def. Jenna Bell (ONU) 6-0, 6-2 |
| R32 | Karen Gao (WASHUW) def. Anna-Maria Huebel (AUGIE) 6-4, 6-3 |
| R32 | Radha Patel (CWR-W) def. Janaki Jagannathan (WOOW) 6-2, 6-2 |
| R32 | Eleanor Archer (WASHUW) def. Lydia Zafeiri (OBEW) 6-4, 6-1 |
| R32 | Oliwia Mikos (CHI) def. Amanda Lopykinski (HOPE) 6-2, 6-3 |
| R32 | Erin Storrer (OTT) def. Dasha Chistyakova (DENW) 6-2, 6-1 |
| R32 | Hannah Kassaie (CWR-W) def. Sarena Biria (CHI) 6-2, 6-3 |
| R32 | Nicole Geller (CHI) def. Otilia Oita (WOOW) 6-0, 6-1 |
| R32 | Penados, Abigail (NCCW) def. Peyton Oda (DENW) 6-2, 6-0 |
| R32 | Nina Moravek (WASHUW) def. Lanie Carder (GRU) 6-0, 6-0 |
| R32 | Eleni Lazaridou (KENW) def. Katalina Wang (CWR-W) 6-2, 6-1 |
| R16 | Sylwia Mikos (CHI) def. Katherine Johnston (WASHUW) 6-2, 6-1 |
| R16 | Lily McCloskey (CWR-W) def. Bassett, Natalie (NCCW) 6-2, 6-0 |
| R16 | Allaire Berl (KENW) def. Shianna Guo (CHI) 7-5, 7-5 |
| R16 | Radha Patel (CWR-W) def. Karen Gao (WASHUW) 6-7 (9-11), 6-3, 6-3 |
| R16 | Oliwia Mikos (CHI) def. Eleanor Archer (WASHUW) 6-2, 6-2 |
| R16 | Hannah Kassaie (CWR-W) def. Erin Storrer (OTT) 6-1, 6-1 |
| R16 | Penados, Abigail (NCCW) def. Nicole Geller (CHI) 0-6, 7-5, 7-5 |
| R16 | Eleni Lazaridou (KENW) def. Nina Moravek (WASHUW) 6-2, 3-6, 6-4 |
| QF | Sylwia Mikos (CHI) def. Lily McCloskey (CWR-W) 6-3, 6-2 |
| QF | Allaire Berl (KENW) def. Radha Patel (CWR-W) 6-3, 6-3 |
| QF | Hannah Kassaie (CWR-W) def. Oliwia Mikos (CHI) 6-3, 0-6, 6-3 |
| QF | Eleni Lazaridou (KENW) def. Penados, Abigail (NCCW) 6-1, 6-1 |
| SF | Sylwia Mikos (CHI) def. Allaire Berl (KENW) 6-3, 6-2 |
| SF | Hannah Kassaie (CWR-W) def. Eleni Lazaridou (KENW) 6-4, 6-0 |
| F | Sylwia Mikos (CHI) def. Hannah Kassaie (CWR-W) 2-6, 6-2, 6-2 |
| C-R32-Q | Kylie Wilson (WIT) def. Sarah Sgambati (JCU) 6-2, 6-1 |
| C-R32-Q | Taylor Goldman (RHIT) def. Kimbeley Peterson (PRIN) 6-1, 6-1 |
| C-R32-Q | Amber Edmonds (WASHUW) def. Borunda, Cristina (MILLIKIN) 6-0, 6-0 |
| C-R32-Q | Addison Brannon (GRU) def. Kimbeley Peterson (PRIN) 6-1, 6-0 |
| C-R32-Q | Lillia Walter (OTT) def. Caroline Lopez (DENW) 6-1, 6-1 |
| C-R32-Q | Katherine Taylor (TRANSY) def. Katie King (DPUW) 2-6, 6-3, 10-4 |
| C-R32-Q | Madeline Kavenagh (JCU) def. Maddie Hurley (KAL) 6-2, 6-2 |
| C-R32-Q | Olivia Colby (FRAN) def. Emilee Bassett (TRINE) 3-6, 6-2, 10-6 |
| C-R32-Q | Ella Frantz (AU-W) def. Melaina Tippery (HAN) 6-2, retired |
| C-R32-Q | Valerie Doherty (DPUW) def. Margaret Bugnacki (LFC) 6-2, 6-3 |
| C-R32-Q | Shadia Amado Aguad (KENW) def. Wynne Aldrich (RHIT) 6-1, 6-3 |
| C-R32-Q | Juliana Pullen (KAL) def. Jamie Hurst (ONU) 7-5, 5-7, 11-9 |
| C-R32-Q | Maria Herrera (FRAN) def. Grace Gardner (TRANSY) 6-1, 6-1 |
| C-R32-Q | Caroline Sproule (WIT) def. Markus, Emily (MILLIKIN) 6-2, 6-2 |
| C-R32-Q | S. Hernandez Cisnero (PRIN) def. Rachel Pinnick (HAN) 6-0, 6-0 |
| C-R32-Q | Megan Long (AU-W) def. Elina Locane (TRINE) 6-1, 4-6, 10-8 |
| C-R16 | Taylor Goldman (RHIT) def. Kylie Wilson (WIT) 6-4, 6-4 |
| C-R16 | Amber Edmonds (WASHUW) def. Addison Brannon (GRU) walkover |
| C-R16 | Katherine Taylor (TRANSY) def. Lillia Walter (OTT) 6-3, 6-2 |
| C-R16 | Madeline Kavenagh (JCU) def. Olivia Colby (FRAN) 6-3, 6-2 |
| C-R16 | Valerie Doherty (DPUW) def. Ella Frantz (AU-W) 6-0, 6-2 |
| C-QF | Shadia Amado Aguad (KENW) def. Juliana Pullen (KAL) walkover |
| C-QF | Maria Herrera (FRAN) def. Caroline Sproule (WIT) 6-3, 7-5 |
| C-QF | S. Hernandez Cisnero (PRIN) def. Megan Long (AU-W) 6-2, 6-3 |
| C-SF | Amber Edmonds (WASHUW) def. Madeline Kavenagh (JCU) 6-2, 6-0 |
| C-SF | Shadia Amado Aguad (KENW) def. Maria Herrera (FRAN) 6-3, 6-4 |
| C-F | Amber Edmonds (WASHUW) def. Shadia Amado Aguad (KENW) 6-4, 4-6, 11-9 |
| R16 | Felicity Kolb (JCU) def. Taryn Simmers (OTT) 8-3 |
| R16 | Lalasa Nagireddy (KENW) def. Claire Loomis (WIT) 8-0 |
| R16 | Madison Wei (HOPE) def. Haley Straub (TRINE) 8-0 |
| R16 | Claire Cardie (FRAN) def. Ellie Bruce (DPUW) 8-3 |
| R16 | Chloe Ku (CWR-W) def. Eleni Bougioukou (KAL) 8-1 |
| R16 | Amelia Bronfman (OBEW) def. Natalee McCann (EC) 8-5 |
| QF | Kristen Ahn (LFC) def. Felicity Kolb (JCU) 6-4, 6-3 |
| QF | Madison Wei (HOPE) def. Lalasa Nagireddy (KENW) retired |
| QF | Chloe Ku (CWR-W) def. Claire Cardie (FRAN) 6-1, 7-5 |
| QF | Randi Hendricks (WASHUW) def. Amelia Bronfman (OBEW) 6-3, 6-0 |
| SF | Kristen Ahn (LFC) def. Madison Wei (HOPE) 2-6, 7-6 (7-3), 10-7 |
| SF | Chloe Ku (CWR-W) def. Randi Hendricks (WASHUW) 6-3, 3-6, 10-4 |
| F | Chloe Ku (CWR-W) def. Kristen Ahn (LFC) 7-5, 1-6, 10-3 |
| C-QF | Claire Loomis (WIT) def. Haley Straub (TRINE) 8-2 |
| C-QF | Eleni Bougioukou (KAL) def. Ellie Bruce (DPUW) 8-6 |
| C-SF | Taryn Simmers (OTT) def. Claire Loomis (WIT) 8-6 |
| C-SF | Natalee McCann (EC) def. Eleni Bougioukou (KAL) 8-6 |
| C-F | Eleni Bougioukou (KAL) def. Taryn Simmers (OTT) 8-1 |
| R16 | Veronica Volkart (PRIN) def. Sara Lopez (LFC) 8-3 |
| R16 | Katie Finerman (WASHUW) def. Evelyn Deren (JCU) 8-1 |
| R16 | Sophia Pischalko (WIT) def. Bailey Higgins (WEB) 8-0 |
| R16 | Miyako Coffey (HOPE) def. Elizabeth Hoff (EC) 8-0 |
| R16 | Madeline Chappars (KENW) def. Olivia Wolfe (KAL) 8-5 |
| R16 | Cassady Shough (OTT) def. Bella DeNardo (DPUW) 8-4 |
| QF | Elsa Syed (CWR-W) def. Veronica Volkart (PRIN) 6-1, 6-0 |
| QF | Katie Finerman (WASHUW) def. Sophia Pischalko (WIT) 6-1, 6-2 |
| QF | Miyako Coffey (HOPE) def. Madeline Chappars (KENW) 6-4, 6-3 |
| QF | Kendall Schrader (DENW) def. Cassady Shough (OTT) 6-1, 6-1 |
| SF | Elsa Syed (CWR-W) def. Katie Finerman (WASHUW) 6-1, 6-3 |
| SF | Miyako Coffey (HOPE) def. Kendall Schrader (DENW) walkover |
| F | Elsa Syed (CWR-W) def. Miyako Coffey (HOPE) 6-3, 6-0 |
| C-QF | Evelyn Deren (JCU) def. Bailey Higgins (WEB) 8-6 |
| C-QF | Olivia Wolfe (KAL) def. Elizabeth Hoff (EC) 8-2 |
| C-SF | Evelyn Deren (JCU) def. Sara Lopez (LFC) 8-1 |
| C-SF | Bella DeNardo (DPUW) def. Olivia Wolfe (KAL) 8-1 |
| C-F | Evelyn Deren (JCU) def. Bella DeNardo (DPUW) 8-2 |
| R16 | Ariana Rodriguez (DENW) def. Kylie Chitwood (WEB) 8-3 |
| R16 | Payne, Kensington (NCCW) def. Claire Hannaford (AU-W) 8-1 |
| R16 | Ana Brand (KENW) def. Abby Cruser (ONU) 8-3 |
| R16 | Alexis Maloney (TRINE) def. Isabella Roberts (HAN) 8-0 |
| R16 | Rena Lin (CHI) def. Maria Hayes (TRANSY) 8-1 |
| R16 | Maddie Cherry (EC) def. Abby Aitken (WOOW) 8-1 |
| QF | Asha Shukla (CWR-W) def. Ariana Rodriguez (DENW) 6-1, 6-0 |
| QF | Ana Brand (KENW) def. Payne, Kensington (NCCW) 6-4, 7-6 (7-5) |
| QF | Rena Lin (CHI) def. Alexis Maloney (TRINE) 6-0, 6-0 |
| QF | Carolyn Tsung (WASHUW) def. Maddie Cherry (EC) 7-5, 6-0 |
| SF | Ana Brand (KENW) def. Asha Shukla (CWR-W) 6-2, 6-2 |
| SF | Rena Lin (CHI) def. Carolyn Tsung (WASHUW) 6-3, 6-3 |
| F | Rena Lin (CHI) def. Ana Brand (KENW) 6-1, 6-2 |
| - | RED - CONSOLATION |
| C-QF | Abby Cruser (ONU) def. Claire Hannaford (AU-W) 8-0 |
| C-QF | Maria Hayes (TRANSY) def. Isabella Roberts (HAN) 8-2 |
| C-SF | Abby Cruser (ONU) def. Kylie Chitwood (WEB) 8-1 |
| C-SF | Maria Hayes (TRANSY) def. Abby Aitken (WOOW) 8-1 |
| C-F | Abby Cruser (ONU) def. Maria Hayes (TRANSY) walkover |
| - | YELLOW |
| R16 | Lauren Richmond (WOOW) def. Allyssa Rassenfoss (TRANSY) 8-3 |
| R16 | Anna Kan (CWR-W) def. Bozovic, Anastasia (NCCW) 8-2 |
| R16 | Julianne Sheldon (ONU) def. Veronica Saunders (FRAN) 8-2 |
| R16 | Hannah Colbert (AUGIE) def. Sullivan Moran (HOPE) 8-6 |
| R16 | Lauren Khou (CHI) def. Payton Dugan (EC) 8-2 |
| R16 | Caroline Kittle (DENW) def. Lauren Dell'Acqua (KENW) 8-2 |
| QF | Brooke Pedersen (CHI) def. Lauren Richmond (WOOW) 6-0, 6-0 |
| QF | Anna Kan (CWR-W) def. Julianne Sheldon (ONU) 6-2, 6-2 |
| QF | Lauren Khou (CHI) def. Hannah Colbert (AUGIE) 6-2, 6-0 |
| QF | Emily Chiasson (WASHUW) def. Caroline Kittle (DENW) 6-1, 6-1 |
| SF | Brooke Pedersen (CHI) def. Anna Kan (CWR-W) 6-4, 6-0 |
| SF | Emily Chiasson (WASHUW) def. Lauren Khou (CHI) 2-6, 7-5, 10-2 |
| F | Brooke Pedersen (CHI) def. Emily Chiasson (WASHUW) 7-6 (7-2), 1-6, 10-7 |
| - | YELLOW - CONSOLATION |
| C-QF | Bozovic, Anastasia (NCCW) def. Veronica Saunders (FRAN) 8-1 |
| C-SF | Bozovic, Anastasia (NCCW) def. Allyssa Rassenfoss (TRANSY) 8-2 |
| C-SF | Payton Dugan (EC) def. Lauren Dell'Acqua (KENW) walkover |
| C-F | Payton Dugan (EC) def. Bozovic, Anastasia (NCCW) 8-2 |
